Albizia lebbeck Benth. is known as the East Indian Walnut in Europe. A. lebbeck plantations are found in Hawaii and different parts of Florida in the United States. It is an erect deciduous tree of Mimosaceae with umbrella shaped crown and is preferred as a timber yielding ornamental tree. Large -scale plantation forestry and afforestation of degraded forestlands are the solution to ensure future sufficiency in biomass. Tissue culture is a very useful and reproducible technique for mass clonal propagation. We used leaf explants on modified MS (Murashige and Skoog 1962) medium to establish in vitro culture. On MS medium modified with 6, BAP (0.4 mg/l), Kinetin (0.25 mg/l) and coconut milk 12% (v/v) the leaflet explants produced callus from the cut end of the explants. We used 0.5% PVP to reduce and dissolve the polyphenol exudation from the explant. The calli could be classified into 4 major types: friable white callus, friable white and green mixed callus, compact green callus and very compact green callus. We found friable white and green mixed callus as the most suitable one for establishing the suspension culture in the liquid medium. We tried different concentrations and combinations of growth regulators ( 6, BAP, Kinetin, 2,4-D, IAA, NAA) and growth supplements (coconut milk, casein hydrolysate) to induce morphogenesis in the suspension culture. MS medium with 6, BAP (8 mg/l), IAA (0.25 mg/l) and coconut milk 8% (v/v) produced embryoids. The embryos followed rhizogenesis and cauliogenesis on agarified medium. We fixed the tissue in 4% Glutaraldehyde and observe the regeneration with SEM after Critical Point Drying and gold plating. Karyotyping was done from well scattered metaphase plate from root tip squash. A pre exposure of the plantlets to the Knop's medium increased the survival efficiency. 66% of the transferred plantlets survived in the greenhouse. The regenerated plants showed normal fast growth and reach the roof of the greenhouse (20 ft.) within 6 months.

Key words: Albizia lebbeck Benth., Karyotyping, SEM studies, Tissue culture