KIM, SANGTAE1,2*, CHONG-WOOK PARK1, and YOUNGBAE SUH2. 1School of Biological Sciences, Seoul National University, Seoul 151-742, Korea; 2Natural Products Research Institute, Seoul National University, Seoul 110-460, Korea. - Phylogeny and evolution of the Magnoliaceae implied by sequences of 10 chloroplast DNA regions.
Phylogenetic analyses of 10 chloroplast DNA regions including
ndhF, rbcL, matK, ORF350, trnL intron,
trnL–trnF, trnH–psbA, rbcL–atpB, trnK 5’
intron, and trnK 3’ intron from 48 selected taxa were carried
out to address phylogenetic questions in the family Magnoliaceae.
Eleven major clades were recognized with strong support in the
subfamily Magnolioideae: (1) Michelia–Elmerrillia–sect.
Maingola–sect. Alcimandra–sect. Aromadendron, (2)
subgenus Yulania, (3) Pachylarnax–sect.
Manglietiastrum–sect. Gynopodium, (4) Kmeria, (5)
sect. Theorhodon sensu stricto (excluding sect.
Splendentes which was recently separated from sect.
Theorhodon)–sect. Magnolia, (6) sect.
Gwillimia–sect. Lirianthe–sect. Blumiana, (7)
sect. Talauma–sect. Splendentes, (8) Manglietia,
(9) sect. Rytidospermum sensu stricto (excluding Magnolia
fraseri, M. macrophylla, and M. dealbata)–sect.
Oyama, (10) M. fraseri, and (11) M.
macrophylla–M. dealbata. Groupings were basically identical
to those recognized in the previous ndhF study. However, the
support for each clade was significantly increased in the extended
data matrix based on 10 cpDNA regions. The time of divergence for
major lineages was estimated, and the maximum rate of nucleotides
substitution for overall 10 cpDNA regions was calculated to be
3.28×10–10 nucleotide substitutions per site per year. The
molecular data strongly suggest that a taxonomic realignment of
infra-familial delimitations and compositions should be considered.
Key words: cpDNA, Magnoliaceae, phylogeny, sequences