SALAZAR, GERARDO A.1,2, MARK W. CHASE1, and MARTIN INGROUILLE2.* 1Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3DS, U.K.; 2Department of Biology, Birkbeck College, University of London, Malet Street, London, WC1E 7HX, U.K.. - Phylogenetic assessment of subtribe Spiranthinae and tribe Cranichideae (Orchidaceae) from four plastid and one nuclear DNA sequence datasets.
We discuss the phylogeny of the predominantly Neotropical subtribe
Spiranthinae, and more broadly of tribe Cranichideae, on the basis of
cladistic analysis of 90 sequences each of two protein-coding plastid
genes (rbcL and matK), two non-coding plastid regions
(trnL intron and trnL-F intergenic spacer), and the
nuclear ribosomal ITS region. These include representatives of all the
subtribes currently recognized as belonging in Cranichideae (except
the monotypic Manniellinae) and representatives of tribes Diurideae
(identified by independent works as sister to Cranichideae), Orchideae
(including Diseae), and Codonorchideae (outgroups). The five datasets
produced highly congruent patterns of relationship and were thus
analyzed in combination. This analysis confirms the close relation of
both Chloraeinae and Pterostylidinae to the "core"
Cranichideae indicated by previous studies of rbcL (Cameron et
al., 1999; Kores et al., 1997) and matK sequences (Kores et
al., 2000), with Chloraeinae sister to a clade including
Pterostylidinae plus Megastylis and the core Cranichideae.
Within the latter, Pachyplectroninae are strongly supported as sister
to a monophyletic Goodyerinae, and this clade is sister to the rest of
Cranichideae. Spiranthinae as delimited by Dressler (1993) are not
monophyletic unless Galeottiella is excluded, and Prescottiinae
are a paraphyletic grade with Cranichidinae embedded in them.
Key words: coding and non-coding plastid sequences, ITS, Orchidaceae, Phylogeny, Spiranthinae