ZHANG, WEN-HENG, ZHI-DUAN CHEN*, HU-BIAO CHEN, and YAN-CHENG TANG. Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China; Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China. - Phylogeny of the Dipsacales s.l. based on chloroplast trnL-F and ndhF sequences.
The phylogeny of the order Dipsacales s.l. is still problematic. Prior
molecular studies based on rbcL and ndhF genes discussed
the phylogeny of Dipsacales s.l., but because of limited sampling the
problems could not be resolved contently. In our research, the
strategies of dense sampling and combined DNA sequence analysis
(trnL F region and ndhF gene) are carried out within
Dipsacales s.l. to elucidate the delimitation of families and the
relationships among them. Sequence data for the trnL-F region
of 26 taxa (25 ingroups and 1 outgroup) and ndhF gene of 28
taxa (25 ingroups and 3 outgroups) were used in the phylogenetic
analyses. Parsimony analysis for the combined data sets for both genes
resulted in only one most parsimonious tree. The results clearly
demonstrate Triplostegia is extremely close to Dipsacaceae;
Morina, which seems nearby Dipsacaceae, is possibly a separate
family of its own. Both Sambucus and Viburnum have very
close relationships with Adoxaceae, which implies they are two members
of that family. Caprifoliaceae s.l. (excluding Sambucus and
Viburnum), which comprises the three clades Linnaeaceae
(Abelia, Dipelta, Kolkwitzia, Linnaea),
Diervillaceae (Weigela) and Caprifoliaceae s.str.
(Heptacodium, Leycesteria, Lonicera,
Symphoricarpos, Triosteum), is paraphyletic. The most
parsimonious tree recognizes seven major clades of Dipsacales s.l.
with the following relationships: (Apiales (Adoxaceae (Diervillaceae
(Caprifoliaceae s.str. (Linnaeaceae (Morinaceae (Dipsacaceae,
Valerianaceae)))))).
Key words: Adoxaceae, Caprifoliaceae s.l., Dipsacales s.l., ndhF, phylogeny, trnL-trnF